Molecular cloning of <i>esr1-1</i>.

<p>(a) Fine mapping of <i>esr1-1</i> with recombination events from 1040 plants analysed for each marker shown. (b-c) Wild-type (WT) and <i>esr1-1</i> genomes were sequenced and inspected for SNP differences within the mapped loci to identify 3 candidate genes. Molecular complementation of the <i>esr1-1</i> mutation by the wild-type <i>At5g53060</i> gene with (b) images and (c) luciferase quantification shown (P<0.05, all pairs Student’s t-test). (d-e) Genetic complementation between <i>esr1-1</i> and a <i>At5g53060</i> T-DNA knockout (SALK_095666) with (d) images and (e) luciferase quantification shown (<i>P</i><0.05, all pairs Student’s t-test). The <i>esr1-2</i> mutant is an F₃ line from a cross between WT plants and the T-DNA insertion line SALK_09566 and is homozygous for the T-DNA insertion and <i>GSTF8</i>:<i>LUC</i> transgene. (f) Structure of the <i>At5g53060</i> gene with <i>esr1-1</i> mutation and T-DNA knockout locations indicated. Filled boxes indicate exons, joining lines indicate introns. Positions are relative to the start codon.