Map-based cloning of <i>YL-1</i> gene.

<p><b>A-B</b>, Position of the <i>YL-1</i> locus in an 3.7 cM interval on Chr. 1 between SSR markers RM10605 and RM10644. <b>C</b>, <i>YL-1</i> was narrowed to a 44.8 kb region between the SSR marker RM10622 and Indel marker Indel 18. <b>D</b>, 4 putative ORFs in the 44.8 kb genomic region. <b>D</b>, Gene structure of the <i>YL-1</i> candidate gene (ORF1). The black boxes represent exons, the lines between them represent introns, and the white boxes means 5,UTR and 3,UTR of <i>YL-1</i>. <b>F-I</b>, Phenotype of wild-type, <i>yl-1</i> and T₁ plant of transgenic <i>yl-1</i>expressing <i>YL-1</i> at the seedling (<b>F</b>) and heading (<b>G-I</b>) stages. Hygromycin resistance detection (<b>J</b>) and qRT-PCR of <i>YL-1</i> (<b>K</b>). <b>L-M</b>, Chlorophyll content (<b>L</b>) and chloroplast ultrastructures (<b>M</b>) in cells of third leaf of wild-type, <i>yl-1</i> and T₁ transgenic plants expressing <i>YL-1</i>. Data in (<b>K</b>, <b>L</b>) are shown as means ± SD from 5 individual replicates.