SBP1 decreases soft agar colony formation in HCT116 cells.

<p>The ability of HCT116 cells with and without SBP1 to form colonies in soft agar was measured. Cells induced to express SBP1 as well as SBP1-null cells were mixed with media containing 0.4% agarose, and each group was plated in triplicate on 6 well plates coated with 0.6% agarose in media. Each well contained 5x10⁴ cells, and colonies larger than 0.5mm were counted on day 15 of growth (A). Error bars represent S.D. (*p<0.05 n = 3).