Let-7c inhibits colony forming abilities of human PCa cells.

<p>Clonogenic (<b>A</b>) and soft agar colony forming (<b>B</b>) abilities of LNCaP-S17 and C4-2B cells transfected with let-7c or empty vector (Con) were assayed. Let-7c inhibited cell growth and survival in anchorage-dependent and –independent conditions. <b>C</b>) Clonogenic assay-Upper and lower panels represent colony sizes of C4-2B and LNCaP-S17 cells expressing control (empty vector) or let-7c respectively. <b>D</b>) Soft agar assay-Upper and lower panels represent colony sizes of C4-2B and LNCaP-S17 cells expressing control (empty vector) or let-7c respectively. <b>E</b>) Number of colonies formed in clonogenic assay by C4-2B cells stably expressing let-7c. Let-7c decreased the number of colonies formed by the PCa cells. Data points represent mean ± SD of triplicate samples from two independent experiments. Error bars denote ± SD (*<i>p</i> < 0.05).