Molecular phylogenetic analysis between 17 sequence types (STs) of 128 pathogenic <i>Leptospira</i> strains based on neighbor-joining (N-J) tree method.

<p>Phylogenetic relationships based on concatenated sequences of 7-locus MLST scheme (3,102 bp) for the 128 pathogenic strains were inferred using N-J method and 1000 bootstrap replications as implemented in MEGA5. Each bacterial strain is labeled as follows: abbreviation of species name (L.int: <i>L</i>. <i>interrogans</i>, L.kir: <i>L</i>. <i>kirschneri</i> and L. bor: <i>L</i>. <i>borgpetersenii</i>), strain name, isolated time, isolated region and (for the 7-locus MLST scheme) sequence type (ST). The dendrogram displays three major clusters corresponding to 3 <i>Leptospira</i> species analyzed in different colors: Red: <i>L</i>. <i>int</i>; Yellow: <i>L</i>. <i>kri</i>; Blue: <i>L</i>. <i>bor</i>.