Bayesian phylogenetic tree of pathogenic <i>Leptospira</i> species from Mayotte (blue) and Madagascar (green) based on concatenated sequences of five genes (<i>secY</i>, <i>adk</i>, <i>lipL32</i>, <i>lipL41</i> and <i>rrs2</i>, total size: 2215 bp).

<p>The analysis was realized under the GTR+G substitution model. At the nodes, grey and white circles indicate posterior probabilities superior to 0.70 and 0.90, respectively. Strain numbers of cultures produced herein are indicated in parentheses, “K” and “U” designating the sequences obtained from kidney or urine, respectively. Stars indicate lineages common to humans and animals (<i>Rattus rattus</i> or <i>Tenrec ecaudatus</i>). For <i>Homo sapiens</i> str. 200901122, the same sequence type was found in nine cultures from <i>T</i>. <i>ecaudatus</i> (2014TE MDI222, 2014TE MDI224, 2014TE MDI294U, 2014TE MDI295, 2014TE MDI295U, 2014TE MDI306, 2014TE MD308, 2014TE MDI321 and 2014TE MDI321U). For <i>H</i>. <i>sapiens</i> str. 200701204, 200900806, 200901483 and 201003760, the same sequence type found was in seven cultures from <i>R</i>. <i>rattus</i> (2014RR MDI247, 2014RR MDI250, 2014RR MDI251, 2014RR MDI259, 2014RR MDI260, 2014RR MDI284 and 2014RR MDI291). Specimen system: MDI = CRVOI specimen catalogue during field trips to Mayotte; FMNH = Field Museum of Natural History, Chicago; UADBA = Université d’Antananarivo, Département de Biologie Animale, Madagascar; for the other bacterial sequences from <i>H</i>. <i>sapiens</i> and <i>Microgale</i> spp. see Bourhy <i>et al</i>. 2012 [<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0004933#pntd.0004933.ref015" target="_blank">15</a>] and Dietrich <i>et al</i>. 2014 [<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0004933#pntd.0004933.ref004" target="_blank">4</a>]. Museum numbers for <i>Microgale</i> spp.: 575 = UADBA 30869; 588 = UADBA 30289; 590 = UADBA 30291; 1335 = UADBA 32122; 1453 = UADBA 32125; 1467 = UADBA 32101.