The fluorescence emission spectra of the N337A mutant.

<p>(A) The fluorescence emission spectra of the N337A mutant with dihydromyricetin of different concentrations (0–50 μM). (B) The fluorescence emission spectra of the N337A mutant with myricetin of different concentrations (0–50 μM). (C) Fluorescence titrations of dihydromyricetin and myricetin with the N337A mutant. An aliquot amount of dihydromyricetin and myricetin was individually added to the enzyme solution for each <i>K</i>_d. The <i>K</i>_d was obtained by the equation: ΔF = ΔF_max-<i>K</i>_d(ΔF/[compound]). Data points are an average of 2–3 determinations within 10% error.