Calculation of the spectroscopic correction factor.

<p><b>Abbreviations:</b> MUNANA, 2′-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid; 4-MU, 4-methylumbelliferone; RFU, relative fluorescence units.</p>a<p>Fluorescence of MUNANA alone in enzyme buffer at 1.95–2000 µM concentrations (shown in column 1).</p>b<p>Fluorescence of 4-MU alone in enzyme buffer at 19 µM concentration. Mean (± SD) 4-MU fluorescence in enzyme buffer was 10222±90 RFU.</p>c<p>Fluorescence of 4-MU in enzyme buffer at 19 µM concentration in the presence of 1.95–2000 µM MUNANA concentrations (shown in column 1).</p>d<p>Values (in RFU) of MUNANA fluorescence (column 2) subtracted from the values (in RFU) obtained in the mixture of 4-MU and MUNANA (column 4).</p>e<p>Correction factor calculated as the ratio of 4-MU fluorescence (in RFU) in the mixture with MUNANA substrate (column 5) and 4-MU fluorescence (in RFU) alone (mean value from column 3).