Oxidative stress in fatty acid-induced podocyte apoptosis mediated by CD36.

<p>(A) Representative immunofluorescence microscopy of ROS in podocytes (200×). Podocytes were treated with 150 μmol/L palmitic acid for 24 hours with or without pretreatment of SSO or tempol, then intracellular ROS level was measured using 2′,7′- dichlorofluorescein diacetate (DCFH-DA) fluorescent probe (green). (B, C) Podocytes were treated with 150 μmol/L palmitic acid for 24 hours with or without pretreatment using tempol, and apoptosis was measured using flow cytometry analysis. (B) Representative cytograms. (C) Percentage of apoptotic cells. * p < 0.05 vs. control group, # p < 0.05 vs. PA group. Data were expressed as mean ± SD. Ctr: Control group, podocytes were treated with 1% BSA. PA: palmitic acid group, podocytes were treated with 150 μmol/L palmitic acid for 24 hours. PA+DMSO: podocytes were treated with 150 μmol/L palmitic acid for 24 hours after pretreatment with dimethyl sulfoxide (DMSO) for 4 hours. PA+SSO: podocytes were treated with 150 μmol/L of palmitic acid for 24 hours after pretreatment with 50 μmol/L sulfo-N-succinimidyloleate (SSO) for 4 hours. tempol: podocytes were treated with 1% BSA for 24 hours after pretreatment with 0.5 mmol/L of tempol for 2 hours. PA+ tempol: podocytes were treated with 150 μmol/L palmitic acid for 24 hours after pretreatment with 0.5 mmol/L of tempol for 2 hours.