Experimental setup.

<p>(A) In the first experimental setup mice were subcutaneously (s.c.) vaccinated three times with SMG or TLA adjuvanted with GERBU in 10 day intervals. On day 27, an oral TLA booster adjuvanted with cholera toxin (CT) was administered in 3% NaHCO₃ buffer and the experiment was terminated on day 34. Sham-vaccinated mice (sham) were sham-vaccinated with GERBU-PBS and sham-boosted with CT in 3% NaHCO₃. (B) In the subsequent setup mice were systemically vaccinated and orally boosted as described in (A) before infection with 500 <i>T</i>. <i>gondii</i> oocysts in 3% NaHCO₃ buffer on day 34 via gavage (SMG + inf; TLA + inf). Sham-vaccinated/infected mice (sham + inf) were sham-vaccinated with GERBU-PBS and sham-boosted with CT in 3% NaHCO₃ before infection. Sham controls (sham + sham) were sham-vaccinated, sham-boosted and sham-infected receiving 3% NaHCO₃ buffer on day 34. The experiment was terminated on day 83.