Changes in ROS activity with blast conditions and with time following blast exposure:

<p>Animals exposed to different blast pressures and with time following blast exposure at mild condition were analyzed for ROS levels. The conversion of colorless CellROX™ dye to deep red fluorescence in the presence of ROS was captured using the Maestro Optical Imaging System. The total signal intensity from all brain slices together was measured, and the result was normalized per pixel to calculate ROS activity levels for each condition. Data are shown as mean ± s.e.m., <i>n</i> = 3–5 animals, *<i>p</i>≤ 0.05. Statistical significance was calculated using one-way ANOVA with Neuman-Keuls post-test.