Depletion of Ly6G⁺ neutrophils impair growth of lung adenocarcinoma in mice.

<p>(A) Quantitation of orthotopic lung tumors in mice treated with either anti-Ly6G (αLy6G) or control (IgG) antibodies as assessed by bioluminescence (BLI) measurements as a function of time (in days, n = 7 and 6 per group respectively). Arrows indicate days at which antibody was administered. Data represent mean ± SEM. (B) Representative BLI images of animals. Color scale bar depicts the photon flux (photons per second) emitted from these mice at day 22. (C) Representative micro-CT slice images of lungs showing tumors in mice treated with anti-Ly6G or control IgG antibody at day 25. Axial, coronal, and sagittal views shown. Bright objects are high density (bone) and black represents air voids within the animal. Axial images were taken from same relative position in each animal; cross-hair points to the same location in all views. D, dorsal; V, ventral; L, left; R, right. (D) Quantification of lung tumor burden by micro-CT analysis (n = 3 per group) HU, Hounsfield Unit (where -1000 is air, -700 is lung, and +100 to +300 is soft tissue). Data represent mean ± SEM. (E) Flow cytometry scatter plots of peripheral blood showing depletion of Ly6G⁺ cells in anti-Ly6G treated mice as compared with control IgG treated mice at 3 days post-treatment. (F) Flow cytometry analysis of peripheral blood showing numbers (cells per ul of blood) of Ly6G⁺ and CD11b⁺ myeloid cells, CD4⁺ and CD8⁺ T cells, B220⁺ B cells, and Ly6C⁺Ly6G^- monocytes. n = 4 per group. Data represent mean ± SEM.