Klf4 suppressed tumor growth and lung colonization.

<p>(A) Quantification of the weight of the tumor lesions in mice (n = 7) subcutaneously injected with MM189 PB-Klf4 or MM189 PB cells. Bar, SE. *, p<0.05. (B) The representative field for detection of Ki-67 expression by immunohistochemistry under the light microscope with 200× magnification in the left panel. The percentage of positive Ki-67 stain was defined as the intensity of positive nuclei divided by that of the total nuclei in the field. Bar, SE. ***, p<0.001. (C) Representative lung fields of nude mice after the delivery, via tail vein injection, of MM189 cells with ectopic Klf4 expression (MM189 PB-Klf4) or vector controls (MM189 PB). The boxed area in the upper panel was shown with macroscopic view. The upper panel was observed at lower magnification (40×) and the lower was for higher magnification (100×). (D) Quantification of weight of the lung lesions in mice (n = 6) injected with MM189 PB-Klf4 or MM189 PB cells. Bar, SE. *, p<0.05. (E) Quantification of total areas of the tumor lesions in lungs of mice (n = 6) injected with MM189 PB-Klf4 or MM189 PB cells. Bar, SE. *, p<0.05.