Expression patterns of <i>Bdtra</i> and <i>Bddsx</i> in RNAi males.

<p>(A, B) Injection with ds<i>Bdtra</i>_79. (C, D) Injection with ds<i>Bdtra</i>_613. (E, F) Injection with ds<i>Bdtra-2</i>_422. (A, C, E) Detection of the transcripts of <i>Bdtra</i>. (B, D, F) Detection of the transcripts of <i>Bddsx</i>. Abbreviations and notes: <i>Bdtra</i> M and <i>Bddsx</i> M: male-specific splicing forms of <i>Bdtra</i> and <i>Bddsx</i> respectively; <i>Bdtra</i> F and <i>Bddsx</i> F: female-specific splicing forms of <i>Bdtra</i> and <i>Bddsx</i> respectively; <i>a-tub</i>, reference gene a-<i>tubulin</i>. The band between <i>Bddsx</i> M and <i>Bddsx</i> F was a male transcript with the size of 130bp shorter than <i>Bddsx</i> M in 3’UTR. Lane 1: female fly from ds<i>EGFP</i>-injection; Lane2: male fly from ds<i>EGFP</i>-injection; Lane 3, 4, 5: XX-reversed males; Lane 6, 7, 8; intersex individuals; Lane M: Takara 100bp DNA ladder.