Expression patterns of <i>Bdtra</i> and <i>Bdtra-2</i>.

<p>(A, B) RT-PCR with <i>Bdtra</i> primers 225+/706- yielded male and female products with different sizes as indicated. RT-PCR with <i>Bdtra-2</i> primers 9+/1185- yielded male and female products of the same size. (C) RT-PCR with male-specific primer ms43+ and non-sex specific primer 706- to amplify the male-specific product. M, 100 bp DNA ladder (Takara); E0–E8, mixed sex embryos collected 0–0.5 h, 0.5–1 h, 1–2 h, 2–4 h, 4–6 h, 6–8 h, 8–10 h, 10–14 h, 14–21h and 24–48 h after egg laying, respectively; L1, first instar larvae of mixed sex; L3, third instar larvae of mixed sex; P, newly pupated pupae of mixed sex; 0d♀,newly emerged female adult; 0d♂, new emerged male adult; ♀md, midguts from 0d♀; ♂md, midguts from 0d♂; ♀fb, fat bodies from 0d♀; ♂fb, fat bodies from 0d♂; ov, ovaries; te, testes; <i>a-tub</i>, reference gene <i>a-tubulin</i>.