qRT- PCR validation of differentially expressed miRNAs supported by Solexa sequencing.

<p>Values shown are Log2(fold-change) in BO versus AO expression levels. qPCR results were normalized to U6 snRNA expression levels, error bars indicate standard errors for replicates (n = 3). The P values are calculated based on a t-test of the replicate values for each miRNA in BO and AO. **means a statistically significant difference (<i>P</i> < 0.01). BO, before onset of the rapid gonad development; AO, after onset of the rapid gonad development.