The abundance of core fucosylation and bisecting modification in N-glycan and glycoprotein levels.

<p>The abundance of core fucosylation and bisecting modification in N-glycan and glycoprotein levels were illustrated using DSA-FACE and serum lectin blot. (A) and (B): The abundance of total core fucosylation residues (the sum of Peak1, 2, 3, 4, 6, 7 and Peak10) from DSA-FACE decreased in LCMM patients (n = 42) than that in healthy controls (n = 42), and decreased according to the severity of the disease [moderate LCMM (n = 14) and severe LCMM (n = 28)]. (C) and (D): LCA binding core-fucosylated glycoprotein level detected by serum lectin blot was decreased in LCMM patients (n = 20) than that in healthy controls (n = 20). The level of serum core-fucosylated glycoprotein was significantly lower in severe LCMM (n = 13) but not in moderate LCMM (n = 7). The vertical axis indicates the ratio of fluorescence intensity of fucosylated proteins to total proteins stained by coomassie blue (CBB). (E) and (F): The abundance of total bisecting residues (the sum of Peak2 and Peak7) from DSA-FACE deceased in LCMM patients than that in healthy controls, and decreased associated with the severity of disease. (G) and (H): PHA-E binding bisecting glycoprotein level detected by lectin blot was decreased in LCMM patients and decreased associated with the severity of disease. The abundance on vertical axis was represented as mean ± standard deviation.