The abundance of total core-fucosylated residues from lens culinaris agglutinin A (LCA) lectin blot, mRNA level of α-1,6-fucosyltransferase (Fut8), guanosine diphosphate (GDP)-fucose transporter (GDP-Tr) from quantitative RT-PCR, and protein level of Fut8 from western blot.

<p>(A) Lectin blots of serum proteins were probed with LCA. The horizontal axis represents the experimental groups: healthy control (n = 30), gastric ulcer (n = 25), and gastric cancer (GC) (n = 30); the vertical axis indicates the ratio of fucosylated proteins to total proteins. The level of fucosylated proteins in gastric cancer is the lowest among 3 groups (P<0.001). (B) Lectin blots from tissue proteins were probed with LCA. The horizontal axis represents the experimental groups: tumor tissues (n = 10) and adjacent tissues (n = 10). The vertical axis indicates the ratio of fucosylated proteins to total proteins. The core-fucosylated level was lower in gastric tumor, but the difference showed no statistical significance (P>0.05). (C) and (D) Relative messenger RNA (mRNA) expression levels of Fut8 or GDP-Tr in tissues were measured by quantitative RT-PCR. The horizontal axis represents the experimental groups: tumor tissues (n = 10) and adjacent tissues (n = 10). The vertical axis indicates the relative expression levels of Fut8 or GDP-Tr. The difference between groups was not statistically significant (P>0.05). (E) The relative abundance of Fut8 protein was illustrated using western blot. The horizontal axis represents the experimental groups: tumor tissues (n = 9) and adjacent tissues (n = 9). The vertical axis indicates the relative levels of Fut8 protein. The relative abundance of Fut8 protein in adjacent tissues was significantly higher than that in tumor tissues (P<0.05).