siRNAs significantly reduce CSE increased SOCE.

<p>A-C. Effect of CSE on hASM cell proliferation. A) Using fluorescent Cyquant proliferation assay, 24 hour exposure to 1- and 2% CSE induced significant cell proliferation when compared to control. Data shown as percent of control (n = 6 patients, minimum duplicate experiments per patient, * = <i>P</i> < 0.001). B) Representative Western blots demonstrating increase in both Cyclin E and PCNA which are useful markers of cell proliferation. C) Cumulative Western blot analysis on hASM cell lysates confirms proliferative effects of CSE through increased expression of Cyclin E and PCNA, known proliferative markers, with CSE exposure when compared to control samples (N = 8 patients, minimum three experiments per patient. Values are means + SE. * = <i>P</i> < 0.005 compared to control, # = <i>P</i> < 0.001 compared to control.