<p>Linear regression analysis of real-time RT-PCR cycle threshold values (Ct, X-axis) and RT-RPA threshold time in minutes (TT, Y-axis) were determined by PRISM (R<sup>2</sup> = 0.39). The RT-RPA is much faster than the real-time RT-PCR even with samples with high Ct value.</p
<p>Ct: cycle threshold; Tt: time threshold; Neg.: Negative; Pos.: Positive; Undet.: Undetermined.</p
Quantitative real-time PCR (QRT-PCR) has been widely implemented for clinical viral load testing, bu...
<p>The 16 collected clinical samples were tested using the TaqMan RT-PCR and RT-PCR, respectively. (...
<p>Forty-five RNA extracts of samples collected from suspected cases of FMDV were screened. Linear r...
A: Threshold time (Y-axis) was plotted versus the log10 concentration of molecules (X-axis) for the ...
<p>A, sample spiked with DENV1; B, DENV2; C, DENV3; D, DENV4. DENV serotypes 1–4 were spiked into pl...
<p>(A) The linear dynamic range of the DENV RT-PCR assay was determined by testing triplicates of 10...
<p>A, DENV1-3 and B, DENV4 RT-RPA assays. Semi-logarithmic regression of the data collected from eig...
<p>Note: The sequences of the primers and probes of CDC DENV-1-4 RT-PCR Assay for detection of DENV...
<p>CDC DENV-1–4 Real Time RT-PCR Assay specificity against clinically relevant concentrations of oth...
Clinical sensitivity and specificity of Mu-RPA compared to real-time PCR for IS2404 (stratified by t...
*<p>AVG CT value is based on one or two samples.</p><p>% CV = coefficient of variation.</p
<p>(A) Scatter plots of FAM and Cy5 drop intensities for a control sample (left) and picoinjected sa...
We compared the performance of a recently established real-time loop-mediated amplification (LAMP) a...
"nFluorescent monitoring of DNA amplification is the basis of real-time PCR. Absolute quantific...
<p>Ct: cycle threshold; Tt: time threshold; Neg.: Negative; Pos.: Positive; Undet.: Undetermined.</p
Quantitative real-time PCR (QRT-PCR) has been widely implemented for clinical viral load testing, bu...
<p>The 16 collected clinical samples were tested using the TaqMan RT-PCR and RT-PCR, respectively. (...
<p>Forty-five RNA extracts of samples collected from suspected cases of FMDV were screened. Linear r...
A: Threshold time (Y-axis) was plotted versus the log10 concentration of molecules (X-axis) for the ...
<p>A, sample spiked with DENV1; B, DENV2; C, DENV3; D, DENV4. DENV serotypes 1–4 were spiked into pl...
<p>(A) The linear dynamic range of the DENV RT-PCR assay was determined by testing triplicates of 10...
<p>A, DENV1-3 and B, DENV4 RT-RPA assays. Semi-logarithmic regression of the data collected from eig...
<p>Note: The sequences of the primers and probes of CDC DENV-1-4 RT-PCR Assay for detection of DENV...
<p>CDC DENV-1–4 Real Time RT-PCR Assay specificity against clinically relevant concentrations of oth...
Clinical sensitivity and specificity of Mu-RPA compared to real-time PCR for IS2404 (stratified by t...
*<p>AVG CT value is based on one or two samples.</p><p>% CV = coefficient of variation.</p
<p>(A) Scatter plots of FAM and Cy5 drop intensities for a control sample (left) and picoinjected sa...
We compared the performance of a recently established real-time loop-mediated amplification (LAMP) a...
"nFluorescent monitoring of DNA amplification is the basis of real-time PCR. Absolute quantific...
<p>Ct: cycle threshold; Tt: time threshold; Neg.: Negative; Pos.: Positive; Undet.: Undetermined.</p
Quantitative real-time PCR (QRT-PCR) has been widely implemented for clinical viral load testing, bu...
<p>The 16 collected clinical samples were tested using the TaqMan RT-PCR and RT-PCR, respectively. (...