Reproducibility of the RPA and real-time PCR assays using data set of 6 runs of serial dilution of the MU molecular standard.

A: Threshold time (Y-axis) was plotted versus the log10 concentration of molecules (X-axis) for the RPA. B: Threshold cycle (CT) on the Y-axis was plotted versus the log10 concentration of molecules (X-axis) for the real-time PCR. RPA assay produced results between 5 to 20 minutes. The error bars represent the range. The real-time PCR had a higher efficiency compared to the RPA, due to the regular cycle format of the PCR, while there is no strict separation of the amplification cycles in the isothermal RPA technology.