Additional file 3: of Overexpression of a bifunctional enzyme, CrtS, enhances astaxanthin synthesis through two pathways in Phaffia rhodozyma

Figure S2. Standard curves for β-actin (a), GAL4 DNA binding domain (DNA-BD) (b) and Estimated copy numbers (PCN) of two plasmids in strains CS19 and CSR19 (C). A 10-fold serial dilution series of pGBKT7-actin, ranging from 1x104 to 1x109 copies/ΟL, was used to construct standard curves using absolute qPCR in triplicate with BDQP and actinQP2 primer sets. Each curve was generated by plotting Cp values against the logarithm of initial template copy number (n= 2). R2: coefficient of determination