10% ethanol-treated MEFs supported hESCs growth.

<p><b>A</b>, Alkaline phosphatase (AKP) staining analysis on hESCs cultured on MEFs treated by different concentrations of ethanol (5%, 10%, 20% and 30%). <b>B</b>, The morphology of hESCs cultured on MEFs treated by different concentrations of ethanol (5%, 10%, 20% and 30%) during growth day 1, 3 and 5. <b>C,</b> AKP staining analysis on hESCs cultured on ethanol-treated MEFs plated with different densities. <b>D</b>, The morphology of hESCs cultured on frozen-thawed eiMEFs. MC, mitomycin C. ET, ethanol. miMEFs, 10 ug/ml mitomycin C inactivated mouse embryonic fibroblasts. eiMEFs, 10% ethanol inactivated mouse embryonic fibroblasts. Red arrows indicate differentiated cells. Scar bar, 100 μm.