Mitotic arrest reveals nuclear-limited Cdc14 release by the FEAR pathway.

<p>See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0128604#sec002" target="_blank">Materials and Methods</a> for the mitotic growth, synchronization and nocodazole-induced arrest procedure. Images of arrested cells were collected 3.5 hours after release from G₁ arrest into nocodazole. Nuclear-localized GFP is shown in green and Cdc14-7Myc protein in red; the white bar represents 5 μm. Cell outlines are shown in solid white, and nuclei in dashed white lines. Cdc14 was scored as nucleolar (filled circles), nuclear (open circles) or cytoplasmic (triangles). (A) Wild-type cells with Cdc14 sequestered in the nucleolus. (B) <i>mad2Δ</i> cells with Cdc14 in the nucleolus. (C) <i>cdc55Δ</i> cells with Cdc14 released into the nucleus only. (D) <i>bub2Δ</i> cells with Cdc14 exported to the cytoplasm. (E) <i>net1-6cdk</i> cells with Cdc14 in the nucleolus. (F) <i>cdc55Δ net1-6cdk</i> cells with Cdc14 in the nucleolus.