Nuclear-limited Cdc14 release during mitosis.

<p>Wild-type cells in log-phase mitosis were stained with DAPI to reveal DNA (in blue) and by indirect immunofluorescence to localize α-tubulin (green) and Cdc14-7Myc (red). Cell cycle stages were identified as described in Materials and Methods. Cell outlines are shown in solid white, and dashed white lines outline the region of strong DAPI-staining (i.e., the nucleus minus the nucleolus, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0128604#sec002" target="_blank">Materials and Methods</a>). The white bar represents 5 μm. (A) A metaphase cell with Cdc14 sequestered in the nucleolus. (B) An early anaphase cell with Cdc14 released to the nucleus. (C) A late anaphase cell with Cdc14 exported to the cytoplasm.