Plasma and liver protein analysis.

<p>NMR, human plasma (30μg) and purified human A2M (2.5μg) were separated by native PAGE (4–20%) (A) and SDS-PAGE (4–20%) under non-reducing (B) and reducing conditions (C). The protein concentration of NMR and human plasma was determined according to Bradford (**—p<0.01) (D). Protein extracts from human and NMR liver (20 μg each) were separated by SDS-PAGE (4–20%) (E) and subjected to western blot analysis using a polyclonal rabbit anti-human A2M antibody (5 μg/mL) and a monoclonal mouse ß-subunit specific anti-human LRP1 antibody (10μg/mL) (F). (A2M –alpha-2 macroglobulin, Alb–albumin, IgG–immunoglobulin, Trf—transferrin).