Western blot of plasma from NMR and human for alpha-2 macroglobulin.

<p>The determination of A2M in plasma samples was performed by electrophoresis in 7% SDS-gel using NMR and human plasma, 30 μg each, and purified human A2M (1–2.5 μg). A2M was quantified by Commassie staining using isolated human A2M to prepare a calibration curve (A). Western blot analyses using a native gradient PAGE (4–20%) was applied to detect the transformed form of A2M using the alpha-1 antibody (B) and a 7% non-reducing SDS-PAGE to detect A2M with a polyclonal antibody using 9 and 3 μg NMR or 3 μg human plasma and 250 ng isolated human A2M (C).