Loss of <i>sgk-1</i> activity induced the UPR-ER.

<p>(A) Fluorescent images of worms carrying a GFP transgene under the <i>hsp-4</i> promoter and treated with control RNAi (empty vector pL4440) or <i>sgk-1</i> RNAi. Scale bars: 50 μm. (B) Quantitation of average pixel intensity of the images shown in (A) and 18 additional images, nine for control RNAi and nine for the <i>sgk-1</i> RNAi. The average intensity is denoted with a red line. There were fifteen L4 worms in each image and ten images for each treatment. *** <i>P</i> value <0.001 (Student’s <i>t</i>-test). (C) Relative mRNA levels of <i>hsp-4</i> in wild type and <i>sgk-1(ok538)</i> mutant animals. ** <i>P</i> value <0.01, * <i>P</i> value <0.05 (Student’s t-test).