Enhanced SIRT3 expression in human gastric cancer cell lines and tumor tissues.

<p>A, SIRT3 protein levels were determined by western blot in 4 gastric cancer cell lines and an immortalized normal gastric epithelium cell line GES-1 (upper panel). SIRT3 protein expression tested in three separate western blots was estimated by measuring the band intensity using Image-Pro Plus software and normalized with β-actin (lower panel). B, SIRT3 mRNA levels were detected by qRT-PCR in 4 gastric cancer cell lines. Data were normalized to immortalized normal gastric epithelium cells. In (A, B), data are presented as mean ± S.E. (n = 3; *, <i>p</i> < 0.05; **, <i>p</i> < 0.01). C, SIRT3 expression in human gastric tumor tissues (n = 29) and adjacent non-tumor tissues (n = 14) was detected by immunohistochemistry staining. SIRT3 expression levels were estimated by density scanning using Image-Pro Plus software and graded as negative, weak positive and strong positive. Scale bar, 50 μm. D, SIRT3 expression in tumor and adjacent non-tumor tissues was presented as percentage of patient specimens. E, SIRT3 expression in intestinal (n = 18) and diffuse (n = 11) types of gastric tumor tissues was presented as percentage of patient specimens.