Expression of SIRT3 in HCC cell lines and tissue samples. A.

<p>mRNA level of SIRT3 in immobilized liver cell line (MiHA) and HCC cell lines was determined by qRT-PCR. Three independent experiments were performed. Data are mean ± SD. <b>B.</b> Representative pattern of SIRT3 protein expressed in cell lines was shown. The ratio of SIRT3/GAPDH was indicated as well. <b>C.</b> mRNA level of SIRT3 in HCC and corresponding adjacent liver tissue was determined in 16 patients. Relative SIRT3 mRNA in HCC tissues was presented. <b>D.</b> Wilcoxon matched paired test revealed the significant alteration of SIRT3 mRNA in tissue samples. <b>E.</b> Expression of SIRT3 protein in 16 paired HCC and adjacent normal liver tissues were examined by western blot. <b>F.</b> Relative intensity of PLK4 normalized to GAPDH was calculated.