<p>(A-C) Proteins from the supernatant of Δ<i>espC</i> strain were fractionated by anion exchange chromatography, using a linear NaCl gradient. (A) Fractions were analysed by Coomassie blue staining. (B) quantification of the EspA and EspD Coomassie stained bands integrated density in (A). (C) anti-EspA and anti-EspD Western blot analysis. Dashed lines indicate editing between lanes from the same gel. EspB eluted in the flow-through. The first peak containing EspA and EspD eluted at 175nM NaCl (peak A/D), the second peak containing EspA eluted at 290 mM NaCl (peak A). (D, E) Fractions corresponding to peak A or peak A/D were incubated with 40 nM of purified EspC for the indicated time. (D) Western blot analysis using the antibodies indicate...
<p>Green fractions from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.001043...
<p>EPEC E2348/69 bacterial cultures were incubated in the presence of CoilA or CoilB peptides and co...
<p>The solubility of PfGAP45-GFP or its mutants and endogenous PfGAP45 was assessed by performing ex...
<p>(A) Confocal micrographs of cells infected for 45 min with the indicated bacteria and processed f...
<p>EPEC strains were grown for 16 h in DMEM to induce T3S. (A, B) Protein contents in supernatant of...
<p>(A) Detection of EspA and EspB that were ectopically expressed and detected by Western blotting u...
<p>(A) Schematic illustration of the expression constructs. (B) Affinity co-elution analysis of Ni<s...
Immunoprecipitation experiments on total protein extracts from the indicated strains were performed ...
Total protein extracts were prepared in triplicate and subjected to Mass Spectrometry analysis. Each...
<p>(A) <i>P</i><sub>r</sub> function of EspB (circles) calculated from SAXS profile and that of phos...
<p>(A) HeLa cytoplasmic lysate was applied to m<sup>7</sup>G-sepharose 4B or sepharose 4B alone. Pre...
<p><b>(A)</b> Western blot of acid-solubilized cell lysates (prepared from bacterial cells grown in ...
A. Immunoblots of pulldown assays using Strep-tactin agarose. EspE-Strep was purified from soluble l...
<p>Cell monolayers were infected with bacteria pretreated with peptides, washed, and lysed for Weste...
<p>Western blots of SDS extracts from exponential cells (see text for details) of strain OG1RF and i...
<p>Green fractions from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.001043...
<p>EPEC E2348/69 bacterial cultures were incubated in the presence of CoilA or CoilB peptides and co...
<p>The solubility of PfGAP45-GFP or its mutants and endogenous PfGAP45 was assessed by performing ex...
<p>(A) Confocal micrographs of cells infected for 45 min with the indicated bacteria and processed f...
<p>EPEC strains were grown for 16 h in DMEM to induce T3S. (A, B) Protein contents in supernatant of...
<p>(A) Detection of EspA and EspB that were ectopically expressed and detected by Western blotting u...
<p>(A) Schematic illustration of the expression constructs. (B) Affinity co-elution analysis of Ni<s...
Immunoprecipitation experiments on total protein extracts from the indicated strains were performed ...
Total protein extracts were prepared in triplicate and subjected to Mass Spectrometry analysis. Each...
<p>(A) <i>P</i><sub>r</sub> function of EspB (circles) calculated from SAXS profile and that of phos...
<p>(A) HeLa cytoplasmic lysate was applied to m<sup>7</sup>G-sepharose 4B or sepharose 4B alone. Pre...
<p><b>(A)</b> Western blot of acid-solubilized cell lysates (prepared from bacterial cells grown in ...
A. Immunoblots of pulldown assays using Strep-tactin agarose. EspE-Strep was purified from soluble l...
<p>Cell monolayers were infected with bacteria pretreated with peptides, washed, and lysed for Weste...
<p>Western blots of SDS extracts from exponential cells (see text for details) of strain OG1RF and i...
<p>Green fractions from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.001043...
<p>EPEC E2348/69 bacterial cultures were incubated in the presence of CoilA or CoilB peptides and co...
<p>The solubility of PfGAP45-GFP or its mutants and endogenous PfGAP45 was assessed by performing ex...