Total protein extracts were prepared in triplicate and subjected to Mass Spectrometry analysis. Each graph shows the abundance of the indicated protein in the three replicates in the various strains. A) EspA. B) EspC. C) EspD. D) EspB. E) WhiB6. WT: H37Rv. Delta_L: ΔespL. Comp_L: ΔespL/espL (complemented strain). Comp_whi: ΔespL/whiB6 (strain expressing whiB6 in trans). Data are expressed as LFQs (Label Free Quantifications).</p
Electrospray ionization mass spectrometry (ESI-MS) is employed to directly analyze the limited tryps...
<p>(A) Immunoblot analysis with anti-Cwp84 antibodies of glycin extracts, showing complete absence o...
<p>Total protein was prepared from colonies of either wild-type, <i>rpoB</i> P564L mutant or Δ<i>rpo...
Total protein extracts were prepared in triplicate and subjected to Mass Spectrometry analysis. Each...
Proteins identified in tandem mass spectromerty analysis of Tloβ2-HA IP experiments (proteins found ...
Proteins identified in tandem mass spectromerty analysis of Tloγ11-HA IP experiments (proteins found...
Volcano plots representing the statistical significance of changes of cell-surface enriched proteins...
Proteins identified in tandem mass spectromerty analysis of Tloα1-HA IP experiments (proteins found ...
Volcano plots representing the statistical significance of changes of the secreted proteins in the s...
<p>(A) EPS as purified from wild-type (WT) or the corresponding mutants by mechanical extraction. EP...
We have developed a strategy to characterize protein isoforms, resulting from single-point mutations...
A) Total cell lysates prepared from the indicated bacterial strains were analyzed by immunoblot. Mem...
<p>A) A schematic representation of the ESX-5 region of <i>M</i>. <i>marinum</i> with the different ...
Additional file 4. SDS-PAGE analysis for soluble and insoluble protein fractions of four clones pres...
<p>Peptide sequences from differentially expressed proteins were identified by mass spectrometry.</p
Electrospray ionization mass spectrometry (ESI-MS) is employed to directly analyze the limited tryps...
<p>(A) Immunoblot analysis with anti-Cwp84 antibodies of glycin extracts, showing complete absence o...
<p>Total protein was prepared from colonies of either wild-type, <i>rpoB</i> P564L mutant or Δ<i>rpo...
Total protein extracts were prepared in triplicate and subjected to Mass Spectrometry analysis. Each...
Proteins identified in tandem mass spectromerty analysis of Tloβ2-HA IP experiments (proteins found ...
Proteins identified in tandem mass spectromerty analysis of Tloγ11-HA IP experiments (proteins found...
Volcano plots representing the statistical significance of changes of cell-surface enriched proteins...
Proteins identified in tandem mass spectromerty analysis of Tloα1-HA IP experiments (proteins found ...
Volcano plots representing the statistical significance of changes of the secreted proteins in the s...
<p>(A) EPS as purified from wild-type (WT) or the corresponding mutants by mechanical extraction. EP...
We have developed a strategy to characterize protein isoforms, resulting from single-point mutations...
A) Total cell lysates prepared from the indicated bacterial strains were analyzed by immunoblot. Mem...
<p>A) A schematic representation of the ESX-5 region of <i>M</i>. <i>marinum</i> with the different ...
Additional file 4. SDS-PAGE analysis for soluble and insoluble protein fractions of four clones pres...
<p>Peptide sequences from differentially expressed proteins were identified by mass spectrometry.</p
Electrospray ionization mass spectrometry (ESI-MS) is employed to directly analyze the limited tryps...
<p>(A) Immunoblot analysis with anti-Cwp84 antibodies of glycin extracts, showing complete absence o...
<p>Total protein was prepared from colonies of either wild-type, <i>rpoB</i> P564L mutant or Δ<i>rpo...