Reverse engineering networks from integrated datasets reveals a novel role of RND3 in cell cycle control.

<p><b>A.</b> Protein-protein interaction network representing a module of RND3 interactors that are co-expressed during tumour development in the CAM. <b>B.</b> Confocal microscopy of U87 cells expressing RND3-GFP with DAPI stain (red). Scale bar: 10μm. <b>C.</b> Immunoprecipitation using anti-Flag antibody in U87 cells transfected with RND3-Flag or Flag. Western blot analyses used the indicated antibodies. <b>D, E.</b> Immunostaining of MCM3 in (<b>D</b>) U87 siControl cells and (<b>E</b>) U87 siRND3 cells. Scale bar: 10μm. <b>F.</b> Quantification of proportion of nuclear and cytoplasmic MCM3 detected by western blot in U87 siRND3 and siControl cells. Data is representative of 3 replicate experiments. *** p < 0.001, ** p < 0.01, * p < 0.05, values +/- SEM.