Rnd3 regulates cell proliferation through NICD signaling in lung adenocarcinoma cells, A549.

<p>(<b>A</b>) Rnd3 mRNA detected by qRT-PCR is down-regulated in A459 compared to HBEC. (<b>B</b>) <b>&</b> (<b>C</b>) A western blot to detect phosphorylated MYPT1, NICD in cells. (<b>D</b>) <b>&</b> (<b>E</b>) The application of compound E blocks the proliferation of A459 cells as detected by a BrdU incorporation assay. The cells were treated with compound E at the final concentration of 5 nM and synchronized by serum depletion followed by growth in media for 12 hours. Then, the cells were treated with BrdU for 30 minutes before being harvested for analysis. (<b>F</b>) <b>&</b> (<b>G</b>) Transient overexpression of Rnd3 in A549 cells down-regulated NICD and Hes1 in a dosage dependent manner. Data represent means ± S.D. *<i>p</i><0.05 compared to control (group 0); #<i>p</i><0.05 compared to group 1; $<i>p</i><0.05 compared to group 3. Data represent means ± S.D.