Inducible expression of <i>KRAS</i>^<i>G12D</i> and <i>TP53</i>^<i>R167H</i> is transforming and tumorigenic.

<p>(<i>a</i>) RT-PCR analysis of <i>KRAS</i>^<i>G12D</i> and <i>TP53</i>^<i>R167H</i> mRNA expression in the fibroblast cell lines from each of the 4 transgenic clones treated with AdCre or AdGFP. (<i>b</i>) Comparison of cell morphology between AdCre and untreated control cells in culture, stained with H&E. (<i>c</i>) Normalized MFU measured by FACS at time points following Carboxyfluorescein succinimidyl ester (CFSE) dye loading of cells. (<i>d</i>) Graphical analysis of the mean number of migrating cells from triplicate plating of each of the 4 cell lines. (<i>e</i>) Graphical analysis of the mean number of colonies growing in soft agar for each cell line from triplicate plating. (<i>c-e</i>: all data points are the mean of the 4 cell lines derived from pigs 63–1, 63–2, 63–3, 63–4; error bars = SD; *p-value ≤ 0.05; **p-value ≤ 0.01).