Phenotypic changes of transformed cells.

<p>A. Morphological properties of transgenic GFF cells. Phase contrast micrographs were shown at 200× magnification. B. Proliferation curves of wild-type GFF cells, LSL-hK-ras^G12D-IRES-HSV1-tk and Lox-hK-ras^G12D-IRES-HSV1-tk GFF cells to indicate enhanced proliferation. 2000 cells per well were seeded in the 96-well plate and incubated for indicated time. The cells were determined by MTT assay. C. Quantification of colony formation between LSL-hK-ras^G12D-IRES-HSV1-tk and Lox-hK-ras^G12D-IRES-HSV1-tk GFF cells. 1, LSL-hK-ras^G12D-IRES-HSV1-tk GFF cells. 2, Lox-hK-ras^G12D-IRES-HSV1-tk GFF cells. Columns, average of five random fields per dish from two dishes; bar, standard deviation (*, p<0.01; chi-square test). D. The anchorage-independent growth of transgenic cells. Cells were suspended in methylcellulose to evaluate anchorage-independent growth potential over 21 days. Bright field micrographs were shown at 100× magnification (2 weeks) and 200× magnification (3 weeks).