Effect of Gln on cell viability and apoptosis.

<p>H9C2 cells were exposed to HG + H/R with different concentrations of Gln (0.5, 1, 2, 4, 8, 16, and 32 mM). (A) Cell viability of each group was determined by MTT assay. (B) Results of the TUNEL assay and DAPI staining of each group. Green spots represented apoptotic bodies and blue spots represent cell nuclei. (C) Bars represent the percentage of TUNEL-positive cells based on the total number of cells stained by DAPI. * <i>P</i> < 0.05 compare with the control group, # <i>P</i> < 0.05 compare with the 1 mM and 4 mM Gln groups. Data are expressed as mean ± S.D. of 3 independent experiments; a.u. = arbitrary units.