Burn Injury Alters the Mouse Intestinal Microbiome.

<p>The non-metric multidimensional scaling (NMDS) plot (<b>A</b>) demonstrates the differences in microbial community structure as a result of burn injury, time since burn injury, and sampling from small and large intestine in a mouse experimental model system of five animals per group. Box-plots of the distribution of the ratio of rRNA genes from Enterobacteriaceae to total bacterial rRNA genes in sham and burn animals, calculated by qPCR, are shown for small and large intestines, one and three days post-burn injury <b>(B)</b>. A statistically significant effect of burn injury was observed between sham and one-day burn injury mice in the small intestine (n = 9 sham, 7 burn mice, two-tailed t-test **, p< 0.01), and between one-day and three-day burn injury mice (n = 9 sham, 8 burn mice, two-tailed t-test *, p< 0.05). A statistically significant effect of burn injury was observed for the large intestine between one-day and three-day burn injury mice (n = 8 animals per group, two-tailed t-test *, p< 0.05). The most abundant bacterial families in sham and burn injury mice (small intestine) are indicated in pie charts <b>(C)</b>, and taxa which were significantly different by Kruskal-Wallis one-way analysis of variance (*, FDR-P <0.05).