Burn injury severely impacts overall health on post burn day 1.

Body weight (A) of mice was determined before and one day after burn or sham injury. Data are presented as weight change in g. Spleen-to-body weight ratios (B) were also determined. Serum G-CSF (C) and serum IL-6 (D) levels of the same mice were quantified by cytometric bead assay. For all experiments, each replicate is plotted (n = 7 mice/group), as well as mean ± SD. Asterisks indicate significant differences as assessed by unpaired, two-tailed Student’s t test: ** p < 0.01; ** p < 0.001.