Morpholino knockdown of <i>agtpbp1</i> and <i>eps15L1</i> results in decreased lymphoid <i>rag1</i> expression.

<p>(A-B) WISH of <i>rag1</i> in 4 dpf control (A) and <i>agtpbp1</i> (B) morpholino-injected embryos. (C) RT-PCR of <i>agtpbp1</i> and <i>ß-actin</i> in pooled control or morphant embryo samples. Quantitation of the normal transcript band normalized to <i>ß-actin</i> is indicated. (D-E) WISH of <i>rag1</i> in 4 dpf control (A) and <i>eps15L1</i> (B) morpholino-injected embryos. (C) RT-PCR of <i>eps15L1</i> and <i>ß-actin</i> in pooled control or morphant embryo samples. Quantitation of the normal transcript band normalized to <i>ß-actin</i> is indicated. Quantitation is in arbitrary units (A.U.), and relative to wild-type level which is set at 1. Head region of the embryos is shown in lateral views, anterior to the left. P values were determined using Fisher’s exact test.