Decreased expression of lymphoid markers in gene-trap mutant embryos.

<p>(A-F) Whole mount RNA in situ hybridization (WISH) of <i>lck</i> in 5 dpf larvae from the lines <i>fcc143</i> (143) (A-B), <i>agtpbp1</i>^{<i>fcc301</i>} (301) (C-D) and <i>eps15L1</i>^{<i>fcc436-P1</i>} (436) (E-F). Representative embryos displaying no GFP (A, E) or normal expression of <i>lck</i> (C) are compared to siblings with strong (str) GFP (B, F) or low levels of <i>lck</i> expression (D). In line <i>agtpbp1</i>^{<i>fcc301</i>}, the GFP expression levels varied, making it difficult to distinguish heterozygous and homozygous carriers. The number of embryos that showed the representative phenotype is indicated in panels C and D.