Hydrophobicity profiles of MPP1 plotted using the Wimley-White scale (top) and the Eisenberg scale (bottom).

<p>The profile for the Wimley-White scale was plotted using Mpex v. 3.2.6. The profile for the Eisenberg scale was plotted using ProtScale. Green areas correspond to identified cholesterol-binding motifs. Dark squares represent residues involved in the formation of salt-bridges. The light-blue graph corresponds to hydrophobic moment. Orange dots represent all of the histidine residues in the MPP1 sequence, which were assumed as uncharged. According to the Wimley-White scale, sequence fragment LPALQMFMR (156–164), apart from its last two residues, is located in a possible membrane-penetration region (indicated by the red line).