Vimentin phosphorylation at other serine residues.

<p>RbCF2 and RbCF8 cells were cultured as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133399#pone.0133399.g001" target="_blank">Fig 1</a>, plated for 30 min followed by treatment in presence or absence of WFA (1 μM) for 1 h. (A) Equal amount of soluble proteins were fractionated by SDS-PAGE and blots were probed with anti-S82vimentin (clone MO82), anti-S71vimentin (clone TM71) and anti-S72vimentin (clone TM72), respectively. Blots were then stripped and re-probed for loading controls. (B) Densitometric quantification of repeat experiments (n = 3; see also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133399#pone.0133399.s001" target="_blank">S1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0133399#pone.0133399.s002" target="_blank">S2</a> Figs) of S82Vim, S72Vim and S71Vim normalized to loading controls, using NIH ImageJ software.