G6 Reduces the Levels of Vimentin Protein within T98G-derived Tumors.

<p>A) Expression of vimentin levels from the same nine tumor samples described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0105568#pone-0105568-g006" target="_blank">Figure 6</a> were determined by Western blot. Also shown are the levels of β-actin which served as a loading control. B) Quantification of vimentin protein levels normalized to β-actin. C) Representative anti-vimentin immuno-histochemistry images showing decreased vimentin expression in the G6 treated tumors. Scale bars represent either 50 µm (top panels) or 10 µm (bottom panels). D) The intensity of vimentin staining was quantified via relative pixel counting and the average pixel counts were then plotted as a function treatment group. E) Tumor sections were subjected to anti-vimentin immune-fluorescence (red) and cell nuclei were visualized via DAPI staining (blue). In response to G6, the remaining vimentin protein formed some irregular perinuclear aggregates. The scale bars represent either 20 µm (large panels) or 5 µm (single inlet panel)*, p<0.05, **, p<0.01.