The activation of different P2Y receptor subtypes reduce <i>T</i>. <i>gondii</i> infection in peritoneal macrophages.

<p>Mouse peritoneal macrophages were infected with <i>T</i>. <i>gondii</i> tachyzoites at a ratio of 5:1 for 2 h and then treated with specific P2Y receptor agonists and antagonists for 30 min, prior to infection index determination. (A) Treatment 2 Thio-UTP at concentrations of 0.05 or 0.1 μM (to activate P2Y₄) and 0.5 or 1 μM of 2 Thio-UTP (to activate P2Y₂) led to similar reductions in the infection index relative to untreated controls. (B) The infection index was also reduced after treatment with MRS 2693, a specific P2Y₆ agonist, and MRS 2693 effect was totally blocked by pre-treatment with the specific P2Y₆ antagonist MRS 2578. The 20, 50, 100 represent on the x-axis concentration of the agonist/antagonist. * statistically significant relative to untreated. # statistically significant relative to reduction of infection index. Data represent mean and SEM of three independent experiments. * p < 0,05; **,^## p < 0.001; ***, ^### p < 0.0001.