Different induction kinetics of ISGs (SOCS1, ISG15, MxA) by type I and type III IFNs.

<p>(A) Induction by 100 IU/mL IFN-α or 50 ng/mL IFN-λ for 0, 1, 4, 8, 16, 24 and 48 hours in Huh-7.5.1. (B) Induction kinetics of ISG15 after stimulation with 100 IU/mL IFN-α or 50 ng/mL IFN-λ in the absence and presence of SOCS1 over-expression. (C) Induction kinetics of MxA after stimulation with 100 IU/mL IFN-α or 50 ng/mL IFN-λ in the absence and presence of SOCS1 over-expression. Total RNA was harvested and reverse transcribed. The levels of mRNA expression were determined by quantitative real time PCR normalized to GAPDH. Data are presented as means ± SEM, n = 3. Error bars indicate standard error of mean (SEM).