Effect of 1, 2, 4 and 5 on the expression of p53 in NCI-H460 and HT-29 cells.

<p>Western blot of p53 and histone H2B (loading control) of NCI-H460 (A) and HT-29 (B) cells. <b>A</b>: Nuclear extracts of NCI-H460 cells, incubated with different concentrations of <b>1</b> (10, 30 and 100 μM) or 10 μM of <b>2</b>, <b>4</b>, or <b>5</b> for 24 hours. Nuclear extracts (NE) and whole cell lysates (WCE) of untreated cells were used as control (Ctrl). <b>B</b>: Nuclear extracts of HT-29 cells, incubated with 10 μM of <b>1</b>, <b>2</b>, <b>4</b>, <b>5</b> for 24 hours. Untreated cells served as control (Ctrl).