Silencing of p53 by specific p53 siRNA inhibited ST-induced G₂ arrest.

<p>Cells were either not transfected or transfected with 100 nM p53 siRNA and then treated with 3 µM ST for 48 h. (A) Cells were subjected to immunoblot analysis for p-p53 (Ser15), p53, p21, and (C) the regulators related to G₂ arrest. NC: cells transfected with the same concentration of negative control siRNA. β-actin was used as the loading control. (B, D) Intensities of the immunoreactive bands in “A” and “C” were quantified by densitometric scanning and compared with those of the control (considered “1”). (E) The cell cycle phases of the cells were analyzed by FCM. The values shown represent the means ± SD, *<i>P</i><0.05 compared with the solvent-treated control group. <b>^▴</b><i>P</i><0.05 compared with the ST-treated groups. ^#<i>P</i><0.05 compared with the p53 siRNA-treated groups.