Characterization of A549-5Ps cells.

<p>(A-B) Stable expression of influenza A virus minigenome transcription/replication system in A549-5Ps cells. A549 and A549-5Ps cells were seeded in 6-well plates and cultured for 48 hours. (A) Gluc activity in supernatants was quantified. (B) Total cellular RNAs were extracted and then subjected to RT-PCR using primer pairs specific for PB2, PB1, NP and model vRNA. (C) Relative Gluc activity over a 96-hour time course at different A549-5Ps cell numbers. A549 and A549-5Ps cells were seeded in 96-well plates (5,000/well, 10,000/well, 20,000/well) and supernatants were harvested at different time points, followed by Gluc activity measurement. Error bars standard error of the mean of three independent experiments.