Additional file 4: of Characterization of the cytolethal distending toxin (typhoid toxin) in non-typhoidal Salmonella serovars

Sequence of cdtB and surrounding region, indicating that deletion is internal to cdtB; cdtB start and stop codons are boxed. Primer sequences in bold. Primer sequence homologous to pKD4 template primer P1 and P2 are italicized. ‘Scar’ sequence from pKD4 in orange. The sequence shown is for the serovar Javiana mutant; the serovar Schwarzengrund and Montevideo mutants showed equivalent sequences. (B) Organization of the region that contains pltB, pltA, and cdtB, which indicates that the cdtB deletion shown in (a) will not disrupt pltA or pltB. (C) Gel that shows the PCR product for the cdtB deletion alleles in serovars Schwarzengrund (lane 1), Montevideo (lane 4), and Javiana (lane 7); these PCR products were used to generate the sequencing data shown in part (A)